Symbiotic efficiency, biosorption and the growth of rhizobia on Horse gram plants under aluminium stress
Prabhavati Edulamudi, Anthony Johnson Anthony Masilamani, Venkata Ramana Sai Gopal Divi, Vishnuvardhan Zakkula, Umamaheswara Rao Vanga, Veera Mallaiah Konada
Abstract
The aim of the present study was to evaluate the tolerance potential
of Horse gram rhizobia to aluminium (Al) toxicity, the enhancement in pod formation,
symbiotic efficiency and biosorption potential in the rhizobia inoculated Horse
gram (
Macrotyloma uniflorum
(Lam.) Verdc.) plants. Initially, 32 isolates of Horse
gram rhizobia were screened for their tolerance of Al in growth media. Among the 32
strains, HGR 4, 6, 13 and 25 that were more tolerant were inoculated individually to
Horse gram plants and the plants were then screened for the ability of pod formation,
symbiotic efficiency and biosorption potential. Among them, maximum pod formation
was observed in Horse gram upon inoculation with HGR-6 and grown at 400 μg
g
-1
of Al. Maximum nodulation was observed in Horse gram upon inoculation with
HGR-6 and HGR-13 grown at 200 μg g
-1
Al. Leghaemoglobin content was maximum
on inoculation with HGR-13 at 400 μg g
-1
of Al. The strain HGR-13 has shown biosorption
potential in soil and as well as in root nodules even at 300 μg g
-1
of Al though
it was maximum at 100 μg g
-1
. This study demonstrated that the Horse gram plants
inoculated with
Rhizobium
strains HGR - 4, 6, 13 and 25, besides having nitrogen fixing
ability also have the ability to grow in Al contaminated soils. Hence, Horse gram
plants associated with these strains of rhizobia could be used in phytoremediation of
metal (Al) contaminated soils.
Keywords
Aluminium, biosorption, metal tolerance, phytoremediation,
Rhizobium
, symbiotic efficiency. |